Whole Genome Amplification:Article Title: Evolutionary-guided advanced deep-learning architecture powers mammalian GPCRome agonist predictions.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies α-sarcomeric actinin Thermo Scientific Cat# MA1-22,863; RRID: AB_557426 WGA (wheat germ agglutinin) Thermo Scientific Cat# MP00831; RRID: AB_2336868 Alexa-Flour 594 labeled secondary antibody Thermo Scientific Cat# A-11005; RRID: AB_141593 DAPI SRL,India Cat# 801451; RRID: AB_2629482 Chemicals, peptides, and recombinant proteins DMSO Sigma Cat# 2650 Isoproterenol Sigma Cat# 5984-95-2 Collagenase Type2 Thermo Scientific Cat17101015 Bovine serum albumin SRL Cat# 85171 PEIMAX 40000 Polyscience Cat# 49553-93-7 DMEM/F-12 medium Thermo Scientific Cat# 12500062 DMEM high glucose medium Thermo Scientific Cat# 12800017 penicillin, and streptomycin Thermo Scientific Cat# 15140122 HTS11217 Maybridge library, CDRI,Lucknow N/A GK03585 Maybridge library, CDRI,Lucknow N/A HTS07541 Maybridge library, CDRI,Lucknow N/A KM07422 Maybridge library, CDRI,Lucknow N/A BTB01595 Maybridge library, CDRI,Lucknow N/A RDP00150 Maybridge library, CDRI,Lucknow NA D-Luciferin GoldBio Cat# 103404-75-7 Deposited data EvOlf Database and Web server This paper https://evolf.ahujalab.iiitd.edu.in/ Project GitHub Page This paper https://github.com/the-ahuja-lab/EvOlf Project Zenodo This paper https://doi.org/10.5281/zenodo.18182113 Experimental models: Cell lines AC16 Cell line Gifted by Dr. Regalla Kumaraswamy, CCMB Hyderabad N/A HEK 293T cell line Takara Cat# 632273 RRID: CVCL_0045 Experimental models: Organisms/strains 1-3-day-old Sprague Dawley (SD) rat pups CSIR CDRI Animal Facility NA Recombinant DNA pGloSensor TM-22F plasmid Gifted by Dr.Prem Naran Yadav, CDRI, Lucknow (Promega) Cat# E2301 β1-adrenergic receptor plasmid Gifted by Dr.Anil Gaikwad, CDRI, Lucknow Addgene Software and algorithms Signaturizer 1.1.13 https://pypi.org/project/signaturizer/ Mordred 1.2.0 https://pypi.org/project/mordred/ Mol2Vec Default GitHub version https://github.com/samoturk/mol2vec MathFeature Default GitHub version https://github.com/Bonidia/MathFeature ProtR 1.7–5 https://cran.r-project.org/web/packages/protr/ vignettes/protr.html (Continued on next page) 16 Cell Reports 45, 117003, March 24, 2026 .. AC16 cells were obtained from Dr. Regalla Kumaraswamy, CSIR-Centre for Cellular and Molecular Biology (CCMB), Hyderabad, who purchased them from Sigma-Aldrich (Cat No. SCC109, RRID: CVCL_4U18).
Labeling:Article Title: Evolutionary-guided advanced deep-learning architecture powers mammalian GPCRome agonist predictions.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies α-sarcomeric actinin Thermo Scientific Cat# MA1-22,863; RRID: AB_557426 WGA (wheat germ agglutinin) Thermo Scientific Cat# MP00831; RRID: AB_2336868 Alexa-Flour 594 labeled secondary antibody Thermo Scientific Cat# A-11005; RRID: AB_141593 DAPI SRL,India Cat# 801451; RRID: AB_2629482 Chemicals, peptides, and recombinant proteins DMSO Sigma Cat# 2650 Isoproterenol Sigma Cat# 5984-95-2 Collagenase Type2 Thermo Scientific Cat17101015 Bovine serum albumin SRL Cat# 85171 PEIMAX 40000 Polyscience Cat# 49553-93-7 DMEM/F-12 medium Thermo Scientific Cat# 12500062 DMEM high glucose medium Thermo Scientific Cat# 12800017 penicillin, and streptomycin Thermo Scientific Cat# 15140122 HTS11217 Maybridge library, CDRI,Lucknow N/A GK03585 Maybridge library, CDRI,Lucknow N/A HTS07541 Maybridge library, CDRI,Lucknow N/A KM07422 Maybridge library, CDRI,Lucknow N/A BTB01595 Maybridge library, CDRI,Lucknow N/A RDP00150 Maybridge library, CDRI,Lucknow NA D-Luciferin GoldBio Cat# 103404-75-7 Deposited data EvOlf Database and Web server This paper https://evolf.ahujalab.iiitd.edu.in/ Project GitHub Page This paper https://github.com/the-ahuja-lab/EvOlf Project Zenodo This paper https://doi.org/10.5281/zenodo.18182113 Experimental models: Cell lines AC16 Cell line Gifted by Dr. Regalla Kumaraswamy, CCMB Hyderabad N/A HEK 293T cell line Takara Cat# 632273 RRID: CVCL_0045 Experimental models: Organisms/strains 1-3-day-old Sprague Dawley (SD) rat pups CSIR CDRI Animal Facility NA Recombinant DNA pGloSensor TM-22F plasmid Gifted by Dr.Prem Naran Yadav, CDRI, Lucknow (Promega) Cat# E2301 β1-adrenergic receptor plasmid Gifted by Dr.Anil Gaikwad, CDRI, Lucknow Addgene Software and algorithms Signaturizer 1.1.13 https://pypi.org/project/signaturizer/ Mordred 1.2.0 https://pypi.org/project/mordred/ Mol2Vec Default GitHub version https://github.com/samoturk/mol2vec MathFeature Default GitHub version https://github.com/Bonidia/MathFeature ProtR 1.7–5 https://cran.r-project.org/web/packages/protr/ vignettes/protr.html (Continued on next page) 16 Cell Reports 45, 117003, March 24, 2026 .. AC16 cells were obtained from Dr. Regalla Kumaraswamy, CSIR-Centre for Cellular and Molecular Biology (CCMB), Hyderabad, who purchased them from Sigma-Aldrich (Cat No. SCC109, RRID: CVCL_4U18).
Recombinant:Article Title: Evolutionary-guided advanced deep-learning architecture powers mammalian GPCRome agonist predictions.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies α-sarcomeric actinin Thermo Scientific Cat# MA1-22,863; RRID: AB_557426 WGA (wheat germ agglutinin) Thermo Scientific Cat# MP00831; RRID: AB_2336868 Alexa-Flour 594 labeled secondary antibody Thermo Scientific Cat# A-11005; RRID: AB_141593 DAPI SRL,India Cat# 801451; RRID: AB_2629482 Chemicals, peptides, and recombinant proteins DMSO Sigma Cat# 2650 Isoproterenol Sigma Cat# 5984-95-2 Collagenase Type2 Thermo Scientific Cat17101015 Bovine serum albumin SRL Cat# 85171 PEIMAX 40000 Polyscience Cat# 49553-93-7 DMEM/F-12 medium Thermo Scientific Cat# 12500062 DMEM high glucose medium Thermo Scientific Cat# 12800017 penicillin, and streptomycin Thermo Scientific Cat# 15140122 HTS11217 Maybridge library, CDRI,Lucknow N/A GK03585 Maybridge library, CDRI,Lucknow N/A HTS07541 Maybridge library, CDRI,Lucknow N/A KM07422 Maybridge library, CDRI,Lucknow N/A BTB01595 Maybridge library, CDRI,Lucknow N/A RDP00150 Maybridge library, CDRI,Lucknow NA D-Luciferin GoldBio Cat# 103404-75-7 Deposited data EvOlf Database and Web server This paper https://evolf.ahujalab.iiitd.edu.in/ Project GitHub Page This paper https://github.com/the-ahuja-lab/EvOlf Project Zenodo This paper https://doi.org/10.5281/zenodo.18182113 Experimental models: Cell lines AC16 Cell line Gifted by Dr. Regalla Kumaraswamy, CCMB Hyderabad N/A HEK 293T cell line Takara Cat# 632273 RRID: CVCL_0045 Experimental models: Organisms/strains 1-3-day-old Sprague Dawley (SD) rat pups CSIR CDRI Animal Facility NA Recombinant DNA pGloSensor TM-22F plasmid Gifted by Dr.Prem Naran Yadav, CDRI, Lucknow (Promega) Cat# E2301 β1-adrenergic receptor plasmid Gifted by Dr.Anil Gaikwad, CDRI, Lucknow Addgene Software and algorithms Signaturizer 1.1.13 https://pypi.org/project/signaturizer/ Mordred 1.2.0 https://pypi.org/project/mordred/ Mol2Vec Default GitHub version https://github.com/samoturk/mol2vec MathFeature Default GitHub version https://github.com/Bonidia/MathFeature ProtR 1.7–5 https://cran.r-project.org/web/packages/protr/ vignettes/protr.html (Continued on next page) 16 Cell Reports 45, 117003, March 24, 2026 .. AC16 cells were obtained from Dr. Regalla Kumaraswamy, CSIR-Centre for Cellular and Molecular Biology (CCMB), Hyderabad, who purchased them from Sigma-Aldrich (Cat No. SCC109, RRID: CVCL_4U18).
Plasmid Preparation:Article Title: Evolutionary-guided advanced deep-learning architecture powers mammalian GPCRome agonist predictions.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies α-sarcomeric actinin Thermo Scientific Cat# MA1-22,863; RRID: AB_557426 WGA (wheat germ agglutinin) Thermo Scientific Cat# MP00831; RRID: AB_2336868 Alexa-Flour 594 labeled secondary antibody Thermo Scientific Cat# A-11005; RRID: AB_141593 DAPI SRL,India Cat# 801451; RRID: AB_2629482 Chemicals, peptides, and recombinant proteins DMSO Sigma Cat# 2650 Isoproterenol Sigma Cat# 5984-95-2 Collagenase Type2 Thermo Scientific Cat17101015 Bovine serum albumin SRL Cat# 85171 PEIMAX 40000 Polyscience Cat# 49553-93-7 DMEM/F-12 medium Thermo Scientific Cat# 12500062 DMEM high glucose medium Thermo Scientific Cat# 12800017 penicillin, and streptomycin Thermo Scientific Cat# 15140122 HTS11217 Maybridge library, CDRI,Lucknow N/A GK03585 Maybridge library, CDRI,Lucknow N/A HTS07541 Maybridge library, CDRI,Lucknow N/A KM07422 Maybridge library, CDRI,Lucknow N/A BTB01595 Maybridge library, CDRI,Lucknow N/A RDP00150 Maybridge library, CDRI,Lucknow NA D-Luciferin GoldBio Cat# 103404-75-7 Deposited data EvOlf Database and Web server This paper https://evolf.ahujalab.iiitd.edu.in/ Project GitHub Page This paper https://github.com/the-ahuja-lab/EvOlf Project Zenodo This paper https://doi.org/10.5281/zenodo.18182113 Experimental models: Cell lines AC16 Cell line Gifted by Dr. Regalla Kumaraswamy, CCMB Hyderabad N/A HEK 293T cell line Takara Cat# 632273 RRID: CVCL_0045 Experimental models: Organisms/strains 1-3-day-old Sprague Dawley (SD) rat pups CSIR CDRI Animal Facility NA Recombinant DNA pGloSensor TM-22F plasmid Gifted by Dr.Prem Naran Yadav, CDRI, Lucknow (Promega) Cat# E2301 β1-adrenergic receptor plasmid Gifted by Dr.Anil Gaikwad, CDRI, Lucknow Addgene Software and algorithms Signaturizer 1.1.13 https://pypi.org/project/signaturizer/ Mordred 1.2.0 https://pypi.org/project/mordred/ Mol2Vec Default GitHub version https://github.com/samoturk/mol2vec MathFeature Default GitHub version https://github.com/Bonidia/MathFeature ProtR 1.7–5 https://cran.r-project.org/web/packages/protr/ vignettes/protr.html (Continued on next page) 16 Cell Reports 45, 117003, March 24, 2026 .. AC16 cells were obtained from Dr. Regalla Kumaraswamy, CSIR-Centre for Cellular and Molecular Biology (CCMB), Hyderabad, who purchased them from Sigma-Aldrich (Cat No. SCC109, RRID: CVCL_4U18).
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